ACB-5000-100 100 test kit
Kit Component - radioimmunoassay
ACB-5001-100 |
Acetylcholine Receptor Reagent-Lyophilized 4 x 1mL |
ACB-5002-100 |
I125 Alpha-Bungarotoxin-lyophilized 4 X 0.75 mL |
ACB-5003-100 |
I125 AChR DILUENT - READY TO USE 4 X 4 mL - 100 Tube Kit |
ACB-5004-100 |
NEGATIVE REFERENCE - normal pool serum. Store at 2-8 C. Contains 0.1 mL of normal human serum |
ACB-5005-100 |
POSITIVE REFERENCE - serum containing AChRAb |
ACB-5006-100 |
Con A-Sepharose - READY TO USE 1 X 5.5 mL Store at 2-8 EC. |
ACB-5007-100 |
NORMAL SERUM - READY TO USE 1 X 4 mL Store at 2-8 EC. For diluting test sera. Contains normal human serum |
ACB-5008-100 |
NSB Reagent 100uL |
ACB-5009-100 |
WASH BUFFER - READY TO USE Tube 2 X 120 mL |
Anti-AChR antibodies are detectable by a radiobinding assay. Specimens and references are incubated for one hour with detergent-solubilized fetal and adult AChRs after that hour I125-alpha-bungarotoxin is added to the tubes, and acetylcholine blocking antibodies directed at the acetylcholine binding cite compete with the I125-alpha-bungarotoxin for a limited number of binding sites on the receptors. The resulting acetylcholine receptor-blocking antibody complexes are then precipitated with Con-A Sepharose. After centrifugation, the supernatant is aspirated and the pellet containing AchR/antibody-bound complex is counted in angamma counter. Counts are inversely proportional to the amount of antibodies present.
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