Free T4 Coated Tube RIA Test Kit

INTENDED USE

The Vet Free T4 Coated TubeRIA Kit is a radioimmunoassay used for determination of free thyroxine in animal plasma or serum.

ANALYTICAL PRINCIPLE

Undiluted samples along with standards and controls are incubated with tracer for 90 minutes in thyroxine antibody coated tubes. During incubation the thyroxine from the samples, standards, and controls compete for binding with the thyroxine from the tracer to the antibody on the surface of the tubes in the presence of blocking agents for thyroid hormone binding proteins. After incubation, the tubes are washed with water to remove unbound thyroxine and tracer. The tubes are read on a gamma counter following wash steps. The level of free thyroxine in the samples, standards, and controls are inversely related to the level of radioactivity that is measured.