Vet Cortisol Coated Tube RIA Test Kit

INTENDED USE

The Vet Cortisol Coated Tube RIA Test Kit is a radioimmunoassay used for the determination of cortisol in animal plasma or serum.

ANALYTICAL PRINCIPLE

Undiluted samples along with standards and controls  are incubated with the tracer for 1.5 hours in a 37 C water-bath in cortisol antibody coated tubes. During incubation the cortisol from the samples, standards, and controls compete for binding with the cortisol from the tracer to the antibody on the surface of the tubes. After incubation, the tubes are washed to remove unbound cortisol and tracer. The tubes are read on a gamma counter following the wash step. The level of cortisol in the samples, standards, and controls are inversely related to the level of radioactivity that is measured.