The Vet Total T3 Coated Tube RIA Test Kit is a radioimmunoassay used for determination of total triiodothyronine in animal plasma or serum.
Undiluted samples along with standards and controls are incubated with the tracer for 1 hour in T3 antibody coated tubes. During incubation the T3 from the samples, standards, and controls compete for binding with the T3 from the tracer to the antibody on the surface of the tubes in the presence of blocking agents for thyroid hormone binding proteins. After incubation, the tubes are washed with water to remove unbound thyroxine and tracer. The tubes are read on a gamma counter following the wash steps. The level of T3 in the samples, standards, and controls are inversely related to the level of radioactivity that is measured.
|Tests per Kit||100/500|
|Method||RIA, Coated Tube|
|Incubation Time||1 hr|