Radioiodination & Purification
We provide I-125 radioiodination and purification services for a wide range of molecules, including proteins, peptides, antibodies, and small molecules. Our services are designed to provide researchers with radiolabeled materials suitable for applications such as binding assays, pharmacokinetic studies, receptor studies, immunoassays, tracer development, and other research applications.
Protein, Peptide & Antibody Iodination
We offer radioiodination services for proteins and other biomolecules containing suitable sites for iodination. Depending on the characteristic and the intended application, different iodination approaches may be used to achieve the desired balance of labeling efficiency, biological activity, and specific activity.
Materials that may be suitable for iodination include:
- Antibodies and antibody fragments
- Proteins and enzymes
- Peptides
- Hormones
- Receptor ligands
- Other biologically active molecules
Following iodination, radiolabeled material is purified to remove unincorporated radioiodine. It can be further purified to remove other components that have not reacted.
Small Molecule Iodination
Small molecules often require a different approach than proteins. We provide custom iodination services for compounds that contain, or can be modified to contain, an appropriate site for radioiodine incorporation.
Depending on the structure of the compound, iodination may involve:
- Direct radioiodination of an appropriate precursor
- Radioiodine exchange reactions
- Oxidative iodination
- Custom synthetic or conjugation approaches
- Labeling through an iodine-containing prosthetic group or intermediate
Purification
Purification is often critical to remove free radioiodine, unlabeled starting material, and unwanted reaction byproducts.
Depending on the molecule and application, we can utilize appropriate purification methods such as:
- Size-exclusion chromatography
- HPLC purification
- Desalting and gel-filtration methods
- Other chromatographic approaches as appropriate
Fractions can be evaluated to identify the desired radiolabeled product and provide purified material suitable for your intended research application.